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Washing your clothes in hot water can indeed significantly reduce the amount of crested wheatgrass pollen. However, it's important to know that hot water is only one part of the process. Temperature alone isn't enough to guarantee complete removal of all pollen grains.
The soap or detergent plays an equally crucial role. Detergents contain surfactants that work by breaking down the outer coating of pollen grains. This allows the water to wash the pollen away more efficiently.
Moreover, the mechanical action of the washing machine helps dislodge pollen embedded in the fibres of your clothes. Therefore, the combination of hot water, detergent, and machine washing is usually the most effective method for removing crested wheatgrass pollen from clothing.
Nevertheless, allergies can still be triggered by small amounts of allergens, and washing machines may not remove 100% of pollen. After washing, the tumble drying process, using a dryer with a good filter, can help further, though air drying indoors is preferable to avoid contact with more pollen outside.
Lastly, remember that it’s not only your clothes that can bring pollen inside. Pollen can also stick to your skin, hair, shoes, and pets, among other things. So, try to ensure you're also keeping these as pollen-free as possible during high pollen season. If you find your allergies persist despite these efforts, consider speaking to your doctor about allergy pills or other treatments.